Investigation of peak purity failure during HPLC method validation led to discovery of a co-eluting impurity under the main peak. Spectral analysis, including three-dimensional modelling, was used to ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

There are a few scenarios where analytical procedures might need to be transferred between HPLC instruments: 1) Method transfer to new equipment in the same lab, perhaps to a different vendor or a ...

An interview with Dr. Michael Dong, about his upcoming talk at Pittcon. Why are stability-indicating methods important in pharmaceutical analysis? The stability-indicating assay is perhaps the single ...

A simple quantitative reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for assay determination of cannabidiol and tetrahydrocannabinol in hemp ...

High Performance Liquid Chromatography (HPLC) is an analytical procedure for separation, identification and quantification of substances using liquid chromatography (LC). The mixture for separation is ...

Green spectrophotometric and HPLC methods have been developed for the quantification of metoclopramide. In the spectrophotometric method, it was determined by direct absorbance measurement at 273 nm ...

High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. Due to its high ...

Analysts interested in organic molecules like dissolved organic matter or pharmaceuticals should consider quantifying organic molecules with the High Performance Liquid Chromatography (HPLC) ...

In the study, the HPLC system was used to analyse the content of various sugars in the plant-based yoghurt alternative. Photo: Lene Hundborg Koss ...

When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...